BDP FL NHS ester是一种在488nm通道的染料，是一种类似于BODIPY FL® NHS ester的分子，还是荧光素的替代剂。用于标记蛋白和多肽的氨基活性染料。 虽然这种荧光染料的吸收和发射光谱比FAM的激发和发射通道范围小，处在其范围内，但BDP FL NHS ester有着更好的耐光性，在亮光下表现突出。但是在单色光分光器上，如果能调整到染料的大发射波长处，将检测到更大信号亮度。 这种染料是中性的，小分子量，在结合物中也能保持高的量子产率。 该染料能够很好的替代fluorescein (FAM), BODIPY-FL, Alexa Fluor 488, DyLight 488, Cy2及其他在488nm纳米处激发的荧光染料。
BODIPY® is a trademark of Life Technologies.
General properties
Appearance:
orange solid
Mass spec M+ increment:
274.1
Molecular weight:
389.16
Molecular formula:
C18H18BF2N3O4
Solubility:
Good in organic solvents (DMF, DMSO, dichlromethane), limited in water
Quality control:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Spectral properties
Excitation maximum, nm:
503
Extinction coefficient at excitation maximum, Lmol-1cm-1:
80000
Emission maximum, nm:
509
Fluorescence quantum yield:
0.9
CF260:
0.015
CF280:
0.027
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