Cyanine5 azide标记试剂用于点击化学,有10mM 溶解于DMSO和粉剂两种形式选择。 该叠氮类染料溶于有机溶剂(如DMSO,DMF),所以标记反应需要有少量有机共溶剂的存在。该染料可用于在水和有机溶剂的混合物中标记炔烃修饰的生物分子。溶解在DMSO中的溶液形式是用于这种生物偶联的即用溶液。水溶的该染料产品:Sulfo-Cyanine5 azide。
Cyanine5是Cy5®的类似物,广泛使用的荧光素之一,能与多种仪器相兼容。Cyanine5可以替代Alexa Fluor 647和DyLight 649。
推荐手册:
寡核苷酸与DNA的点击化学标记
Cy® is a trademark of GE Healthcare.
General properties
Appearance:
dark blue powder / solution
Molecular weight:
601.22
Molecular formula:
C35H45ClN6O
Solubility:
soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Quality control:
NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:
Download
Spectral properties
Excitation maximum, nm:
646
Extinction coefficient at excitation maximum, Lmol-1cm-1:
250000
Emission maximum, nm:
662
Fluorescence quantum yield:
0.2
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