推荐手册：
SYBR Green I 荧光定量PCR
Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
General properties
Appearance:
light orange solution
Quality control:
NMR 1H (95%) and 13C, TLC, PCR testing
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS:
Download
Spectral properties
Excitation maximum, nm:
454
Extinction coefficient at excitation maximum, Lmol-1cm-1:
73000
Emission maximum, nm:
524
Fluorescence quantum yield:
0,8
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