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DIG High Prime DNA Labeling and Detection Starter Kit II

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产品名称: DIG High Prime DNA Labeling and Detection Starter Kit II
产品型号: Roche 11585614910
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DIG High Prime DNA Labeling and Detection Starter Kit II|DNA高效地高辛标记及检测试剂盒II


DIG High Prime DNA Labeling and Detection Starter Kit II  的详细介绍

DIG High Prime DNA Labeling and Detection Starter Kit II

DIG High Prime DNA Labeling and Detection Starter Kit II|DNA高效地高辛标记及检测试剂盒II|地高辛标记试剂盒|高效地高辛标记试剂盒|罗氏地高辛标记试剂盒


DNA高效地高辛标记及检测试剂盒II （Roche 货号： 11585614910）

DIG-High Prime DNA Labeling and Detection Starter Kit II

1 kit for 12 labeling reactions and detection of 24 blots 10 ng to 3 μg per assay, blots of 100 cm2

Application

DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Benefits

The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.

Product Description

Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

Contents

  1. DIG-High Prime, 5x conc., 50 µl
  2. DIG-labeled Control DNA, 20 µl, (5 µg/ml) pBR328 (linearized with Bam HI)
  3. DNA Dilution Buffer, 3 x 1 ml
  4. Anti-digoxigenin-AP Conjugate, 50 µl
  5. CSPD, ready-to-use, 50 ml
  6. Blocking Solution, 10x conc., 4 x 100 ml
  7. DIG Easy Hyb Granules, 4 x 100 ml

DIG High Prime DNA Labeling and Detection Starter Kit II
产品相关关键字： DIG High Prime DNA Labeling and Detection Starter DNA高效地高辛标记及检测试剂盒II 地高辛标记试剂盒 高效地高辛标记试剂盒 罗氏地高辛标记试剂盒

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