DPPIV drug discovery kit Enzo lifescience BML-AK499；二肽基肽酶 4 /二肽基肽酶IV 检测试剂盒，原装进口，现货供应！
The DPPIV Drug Discovery Kit is a complete assay system designed to screen DPPIV inhibitors, providing enough material to perform at least 96 assays. DPPIV (DPP4, CD26) is a member of the class of proteases known as prolyl peptidases, which cleave proteins after proline residues and is thought to play roles in diabetes, cancer, and autoimmune diseases, making it a target for drug discovery.The kit contains both a chromogenic substrate (H-Gly-Pro-pNA; Km=114 µM) and a fluorogenic substrate (H-Gly-Pro-AMC; Km=50 µM).Cleavage of the p-nitroaniline (pNA) from the colorimetric substrate increases absorbance at 405 nm. The fluorimetric assay is based on the cleavage of 7-amino-4-methylcoumarin (AMC) moiety from the C-terminus of the peptide substrate, which increases its fluorescence intensity at 460 nm. The kit is useful to screen inhibitors of DPPIV, a potential therapeutic target. A DPPIV inhibitor, P32/98 (KI=130 nM12), is included for use as a control. Other DPP enzymes are available for specificity profiling.
Calibration standard (7-Amino-4-methylcoumarin) (Prod. No. BML-KI107) (1ml; 40µM in assay buffer) Storage: -70°C)Inhibitor (Prod. No. BML-PI142-9090) (20µl; 1mM in DMSO) Storage: -70°CAssay Buffer (50mM TRIS, pH 7.5) (Prod. No. BML-KI342) (20ml; liquid in screw-cap plastic bottle) Storage: -20 or -70°C½-volume clear microplate (Prod. No. BML-K101)Storage: Room temperature
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